<i>Agrobacterium</i>-mediated transformation of protocorm-like bodies in <i>Dendrobium</i> Formidible ‘Ugusu’
نویسندگان
چکیده
منابع مشابه
Preliminary Factors Influencing Transient Expression of Gusa in Dendrobium Savin White Protocorm-like Bodies (PLBs) Using Agrobacterium-mediated Transformation System
A critical step in the development of Agrobacterium tumefaciens-mediated transformation in orchid genus is the establishment of optimal conditions for T-DNA gene delivery into protocorm-like bodies from which the whole plantlets can be regenerated. As a first step in the development of a successful Agrobacterium tumefaciens-mediated transformation for Dendrobium savin white, factors influencing...
متن کاملAmmonium to Nitrate Ratio Affects Protocorm Like Bodies PLB Formation In vitro of Hybrid Cymbidium
Carbon and nitrogen are the two elements that most affect plant organogenesis. In vitro, usually as part of the macronutrients. Some anecdotal evidence from the literature suggests that the ammonium (NH4+) to nitrate (NO3–) ratio may affect orchid organogenesis. In this study, to test this hypothesis, different NH4+: NO3– ratios were tested on the development of protocorm-like bodies (PLBs) of ...
متن کاملEffects of organic additives and different carbohydrate sources on proliferation of protocorm- like bodies in Dendrobium
The purpose of this study was to investigate the effect of the addition of different organic additives and sugar types as carbon source on the growth and proliferation of protocorm-like bodies (PLBs) of a very new Dendrobium hybrid known as D. Alya Pink (DAP). PLBs were supplemented with homogenates of banana, tomato, and coconut water at various concentrations. Proliferation rate was recorded ...
متن کاملammonium to nitrate ratio affects protocorm like bodies plb formation in vitro of hybrid cymbidium
carbon and nitrogen are the two elements that most affect plant organogenesis. in vitro, usually as part of the macronutrients. some anecdotal evidence from the literature suggests that the ammonium (nh4+) to nitrate (no3–) ratio may affect orchid organogenesis. in this study, to test this hypothesis, different nh4+: no3– ratios were tested on the development of protocorm-like bodies (plbs) of ...
متن کاملEffect of Banana Extract on Growth and Development of Protocorm Like Bodies in Dendrobium sp. orchid
An experiment was carried out in Plant Tissue Culture Laboratory, Department of Crop Botany, Bangladesh Agricultural University, Mymensingh to investigate the effect of banana extract on micropropagation of Dendrobium sp. var. Sonia orchid through PLBs. The experiment was conducted during July 2012 to October 2013. Half-Murashige and Skoog (1/2MS) medium were used as basal medium and the medium...
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ژورنال
عنوان ژورنال: Plant Biotechnology
سال: 2015
ISSN: 1342-4580,1347-6114
DOI: 10.5511/plantbiotechnology.15.0619a